Treatment of diabetic wounds using nanofibrous dressings releasing platelet lysate components
Treatment of diabetic wounds is problematic, long-lasting, expensive and restrictive for patients. Platelets are physiologically present in the process of healing of acute wounds, where they secret growth and angiogenic factors, cytokines, chemokines, cell adhesion-mediating molecules and other bioactive substances that support healing. Lysate prepared from platelet-rich plasma will be incorporated into degradable and non-degradable nanofibrous dressings, prepared from synthetic and natural polymers further modified with fibrin, in order to assure controlled release of growth factors and other bioactive substances. Bioactivity of these dressings will be evaluated in vitro in cultures of human keratinocytes, dermal fibroblasts, vascular endothelial cells and adipose tissue-derived stem cells. Nanofibrous dressings with the best properties will be applied in vivo on wounds of diabetic rats, and their ability to stimulate wound healing will be evaluated.